We can visualize the reads and enriched regions using the Partek Genome Viewer.
- Select ChIP-Seq from the spreadsheet tree
- Select Plot Chromosome View from the Visualization section of the ChIP-Seq workflow
The Genome Viewer will open. The default tracks are the transcript tracks, the sequence visualization tracks, and the cytoband track (Figure 1).
Figure 1. Viewing ChIP-Seq data in the Genome Viewer
- Select New Track from the left-hand side of the Tracks tab
- Select Add tracks from a list of spreadsheets
- Select Next >
- Select p-value_filtered.txt and Motifs_instances.txt tracks
- De-select Aligned Reads
- Select Create
This will display the enriched regions found in the samples and the locations of the motif instances from the de novo motif discovery (Figure 3). The two track display the detected regions at each location on the chromosome for the NRSF-enriched sample, chip, and aligned them to the de novo discovered motif binding sites.
Additional Assistance
If you need additional assistance, please visit our support page to submit a help ticket or find phone numbers for regional support.
| Your Rating: |
|
Results: |
|
0 | rates |
Overview
Content Tools