Partek Flow Documentation

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Data tracks section of the Select tracks dialog enables you to specify the tracks for visualization on the canvas. An overview of the available track types is provided in Figure 1. Note that not all tracks are visible at all times and that their presence depends whether specific data types are present in the project as well as the zoom level. The tracks can be customised customized and their appearance changed by using the control panel on the left. Different track also has it own specific configuration settings which allow you to pin the track to move the track, pin the track, change the styles of the track and hide the track using the settings options on the left of each track ( )

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Alignments track displays a histogram view of alignments present in .bam files in a stacked histogram fashion (similar to Partek® Genomics Suite®). The y-axis shows number of (raw) base calls per position. By default, reads are coloured by sample. Variations on the track are displayed below. They can be configured in the following ways:

  • The histogram can be colored by sample, attribute groups or by base call. By default, it is colored by sample except when the chromosome viewer is invoked from a variant data table. 
  • When colouring reads by sample, the reads are stacked (on top of each other), i.e. in the example below there are more reads in the red sample than in the blue sample. This is an example of a Sum histogram type. This can also be configured to display overlays or averages.
  • Reads can be split into two tracks corresponding to the strand that they map to. This can be invoked by clicking the Split read histogram by strand checkbox.
  • Y-axis can be scaled for all samples to have the same max or each sample has its own specific max

For more information on configuring tracks see our page on Customizing the chromosome viewer

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The Isoform proportion track displays the reads mapped to transcripts and helps to visualize differential expression and alternative splicing, using this track is only available on the feature list data node which is generated from differential expression analysis. It uses standard symbols for exons (boxes) and introns (lines connecting the boxes). The size height and color of each transcript is proportional to the number of reads that map to that transcript. The color indicates the samples to which the reads belong. proprotional to its LS mean value.  Figure 3 shows a gene with two transcripts in RefSeq database; the top transcript is more abundant than the bottom transcript and is preferentially expressed in the "blue" condition (labeled as 0 uM). The bottom transcript, on the other hand, seems to be expressed at the same level across all three conditions (i.e. 0 uM, 5 uM, 10 uM). The number and structure of transcripts on the plot depend on the transcript model that was used for mapping.

For more information on configuring tracks see our page on Customizing the chromosome viewer

 

Numbered figure captions
SubtitleTextIsoform proportion track: the transcripts are shown as present in the transcript model that was used for mapping. Exons are depicted as boxes. The size of each transcript is proportional to the number of reads mapping to it. Colors indicate samples to which the reads belong
AnchorNameIsoform proportion track

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