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Trim tags allows you to process sequences unaligned read data with adaptors, barcodes, and UMIs using a Prep kit file that specifies the structure cofiguration of the adapters, barcodes, UMIs, and insert sequences in your reads. 

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Partek distributes prep kits for processing several types of data - :

• Drop-seq

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• 10x Chromium Single Cell 3' v2

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• Fluidigm C1 mRNA Seq HT IFC

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• Rubicon Genomics ThruPLEX Tag-seq

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If your data is from one of these sources, you can select the appropriate option in the Prep kit drop-down menu. If the data is from another source, you can build a custom prep kit file to process your data.

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The output of Trim tags is a Trimmed reads data node (and an . An additional Untrimmed reads data node will be generated if the Keep untrimmed untrimmed option was selected).

The task report provides a table with the total reads, reads retained, % reads retained, reads removed, and % reads removed for each sample (Figure 2). You can click Download at the bottom of the table to save a text file copy to your computer. 

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The insert is the sequence retained after trimming in the Trimmed reads data node. For example, in RNA-Seq, this would be the mRNA sequence. Every prep kit must include an insert segment. You can specify the minimum size of the insert section using the Length field (Figure 10). 

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