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• Select New Group twice to add two additional groups
• Set Set Attribute name: to hPSC State
• Rename the four groups Primed, Naive, shPOU5F1, and shNANOGPrimed and Naive
• Drag and drop the samples from the Unassigned list to their groups as listed in the table below

There should now be four two groups with two samples in each group (Figure 4).

• Select OK
• Select Yes from the Add another categorical attribute dialog
• Set Attribute name: to shRNA treatment
• Select New Group to add an additional group
  
• Rename the three groups Control, shPOU5F1, and shNANOG
• Drag and drop the samples from the Unassigned list to their groups as listed in the table below

There should now be three groups with samples in each group (Figure 5).

• Select OK
• Select No from the Add another categorical attribute dialog
• Select Yes to save the spreadsheet

A Two new column as been added to spreadsheet 1 (Differential Methylation Analysis) with the experimental group state and shRNA treatment of each sample (Figure 56). 

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