The significant CpG loci detected in the previous step actually form a methylation signature , which we shall now visualise by that differentiates between LCLs and B cells. We can build and visualize this methylation signature using clustering and a heat map. Start by selecting the shPOU5F1_vsPrimed

• Select the LCLs_vs_Bcells_CpG_Islands spreadsheet in the spreadsheet pane on the left

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• Select Cluster Based on Significant Genes from the

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• Visualization panel of the Illumina BeadArray Methylation workflow
• Select Hierarchical Clustering for Specify Method (Figure 1)
  
  

• Select OK
• Verify that LCLs_vs_Bcells_CpG_Islands is selected in the drop-down menu
• Verify that Standardize is selected for Expression normalization (Figure 2)
  
  

• Select OK 

The heat map will be displayed on the Hierarchical Clustering tab (Figure 13).