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Before proceeding to exploratory data analysis, the study samples need to be organized in four groups, two biological replicates each. In the Import section of the workflow, select Add Sample Attributes and, in the next dialog (Figure 2), Add a Categorical Attribute (and then OK).

 

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SubtitleTextAdding sample attributes. Adding Attributes from an Existing Column can be used to split file names into sections, based on delimiters (e.g. _, -, space etc.). Adding a Numeric or Categorical Attribute enables the user to manually specify sample attributes
AnchorNameadd sample attributes selector

 

The following dialog is Create Categorical Attribute (Figure 3). The Attribute name filed specifies the name of the new column (attribute), while the groups (levels of the attribute) are specified in the Group Name fields. By default, two groups are created, but additional ones can be added using the New Group button. Finally, drag and drop the samples from the Unassigned list to their matching groups. 

 

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SubtitleTextCreate Categorical Attribute is used to define new groups and assign samples to them. The name of new column (attribute) is specified in the Attribute name field, group labels (attribute levels) are specified in the Group Name fields. To assign samples to groups, use drag and drop (to select more than one, use Ctrl or Shift buttons)
AnchorNameassigning samples to groups

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