Partek Flow Documentation

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SubtitleTextInvoking Post-alignment QA/QC
AnchorNameInvoking post-alignment QA/QC

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Post-alignment QA/QC task node will be generated (Figure 2). 

 


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SubtitleTextPost-alignment QA/QC task node
AnchorNamePost-alignment QA/QC task node

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  • Double-click the Post-alignment QA/QC task node to view the task report

Similar to the Pre-alignment QA/QC task report, general quality information about the whole data set is displayed and sample-level reports can be opened by clicking a sample name in the table. 

The top two graphs in the data set view (Figure 3) show the alignment breakdown and coverage. Please note that the left axis label on each graph corresponds to the bars and the right axis label corresponds to the lines. 

 


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SubtitleTextAlignment breakdown statistics for the data set showing Alignment percent as bar chart and Total reads as line graph
AnchorNameAlignment breakdown

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From these graphs, we can see that more than 95% of reads were aligned, but the total number of reads for each sample varies. Normalizing for the variability in total read counts will be address addressed in a later section of the tutorial. 

For more information about the graphs and information presented in the Post-alignment QA/QC task report, see the Post-alignment QA/QC user guide. 

 


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