Partek Flow Documentation

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Data tracks section of the Select tracks dialog enables you to specify the tracks for visualization on the canvas. An overview of the available track types is provided in Figure 1. Note that not all tracks are visible at all times and that their presence depends on the whether specific data types are present in the project as well as the zoom level. The tracks can be customised customized and their appearance changed by using the control panel on the right.left. Different track also has it own specific configuration settings which allow you to pin the track to move the track, pin the track, change the styles of the track and hide the track using the settings options on the left of each track (Image Added )

 

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SubtitleTextData tracks in Chromosome view (examples)
AnchorNameData tracks

Alignments track

Isoform proportion track

Variants track

Amino acids track

Reads pileup track

Probe intensities track

Peaks trackImage Added


Alignments Track

Alignments track displays a histogram view of alignments present in .bam files in a stacked histogram fashion (similar to Partek® Genomics Suite®). The y-axis shows number of (raw) base calls per position. By default, reads are coloured by sample; the exception is invocation of the chromosome view on a variant table, when the reads are coloured by base calls. The difference is shown in Figure 2.

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. Variations on the track are displayed below. They can be configured in the following ways:

  • The histogram can be colored by sample, attribute groups or by base call. By default, it is colored by sample except when the chromosome viewer is invoked from a variant data table. 
  • When colouring reads by sample, the reads are stacked (on top of each other), i.e. in the example

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  • below there are more reads in the red sample than in the blue sample. This is an example of a Sum histogram type. This can also be configured to display overlays or averages.

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  • Reads can be split into two tracks corresponding to the strand that they map to. This can be invoked by clicking the Split read histogram by strand checkbox.
  • Y-axis can be scaled for all samples to have the same max or each sample has its own specific max

For more information on configuring tracks see our page on Customizing the chromosome viewer

 

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SubtitleTextVarious configurations of the Alignments track
AnchorNameAlignments track

Reads coloured by sample

Reads coloured by base calls

Reads split by strand

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Isoform Proportion Track

The Isoform proportion track displays the reads mapped to transcripts and helps to visualize differential expression and alternative splicing, using this track is only available on the feature list data node which is generated from differential expression analysis. It uses standard symbols for exons (boxes) and introns (lines connecting the boxes). The size height and color of each transcript is proportional to the number of reads that map to that transcript. The color indicates the samples to which the reads belong. proprotional to its LS mean value.  Figure 3 shows a gene with two transcripts in RefSeq database; the top transcript is more abundant than the bottom transcript and is preferentially expressed in the "blue" condition (labeled as 0 uM). The bottom transcript, on the other hand, seems to be expressed at the same level across all three conditions (i.e. 0 uM, 5 uM, 10 uM). The number and structure of transcripts on the plot depend on the transcript model that was used for mapping.

For more information on configuring tracks see our page on Customizing the chromosome viewer

 

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SubtitleTextIsoform proportion track: the transcripts are shown as present in the transcript model that was used for mapping. Exons are depicted as boxes. The size of each transcript is proportional to the number of reads mapping to it. Colors indicate samples to which the reads belong
AnchorNameIsoform proportion track

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Variants Track

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SubtitleTextVariants track at low power magnification: SNVs are symbolized by purple columns and an insertion is presented as a green bar (an example is shown). A deletion is presented as a red bar (none is visible on the figure)
AnchorNameVariants track at low magnification

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Upon zoom-in, SNVs are drawn as pie charts, representing the proportion of each base call at that locus (Figure 5)

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SubtitleTextVariants track at high power magnification: each SNV is presented as a pie chart and each slice symbolises the relative frequency of each base call (an example is shown). Base call colour codes are given by the track name
AnchorNameVariants track at high power magnification

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At higher modification, insertions are seen as green boxes, with individual inserted bases presented using a pie chart, while deletions look like red boxes and the affected bases are also presented by a pie (Figure 6). 

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SubtitleTextVariants track at high power magnification: insertion is presented as a green box, deletion is presented as a red box. An example is shown.
AnchorNameVariants track at high power magnification

Insertion
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Deletion
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Amino Acids Track

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SubtitleTextAmino acids track at high power magnification: consensus amino acid sequence is at the bottom of the track, while a variant is shown on the top (change from Threonine to Proline is shown)
AnchorNameAmino acids

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If an amino acid spans two exons, its box will be truncated and the line connecting the exons will be dashed. An example is in Figure 8.

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SubtitleTextAmino acids track: exon-spanning amino acids indicated by truncated boxes (i.e. Alanine on the left) (an example is shown)
AnchorNameexon-spanning amino acids track

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An empty gray box on the top of consensus sequence is used to indicate a STOP codon, which is a consequence of a mutation (Figure 9).

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SubtitleTextAmino acids track: A variant which is in fact a STOP codon is represented by an empty box, as seen on the top of the G (an example is shown)
AnchorNameSTOP codon

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Untranslated bases, such as ones downstream of a STOP codon are depicted by lighter shades. Figure 10 shows two transcripts in an amino acid track; the direction is from left to right, so amino acids downstream of a STOP codon (P > G > L) are lightly shaded.

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SubtitleTextAmino acids track: amino acids downstream of a STOP codon are depicted by lighter shades. STOP codon is represented by "." in the middle, direction is from right to left (an example is shown)
AnchorNameDownstream amino acids

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Reads Pileup Track

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SubtitleTextReads pileup track: short sequencing reads are represented as bars. Paired-end reads are located within a gray box encompassing both pairs. Singletons, such as that on the top right, are depicted as thicker reads (an example is shown)
AnchorNameReads pileup track

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If you used a junction-aware aligner (such as TopHat or STAR), the junction reads will be depicted using dashed lines, which connect exon-spanning parts of the same read (Figure 12).

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SubtitleTextReads pileup track: junction reads are depicted using dashed lines. A RefSeq track is added at the top, to visualise the exons (an example is shown)
AnchorNamejunction reads

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Deleted bases can also be seen on a Reads pileup track, as fat black lines (Figure 13).

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SubtitleTextReads pileup track: deleted bases depicted using fat black lines (an example is shown)
AnchorNameDeleted bases

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Probe Intensities Track

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SubtitleTextProbe intensities track: probes are depicted as bars and their colour reflects the intensity (an example is shown)
AnchorNameProbe intensities track

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As with the Reads pileup track, probes may not be visible with low power magnification and you will see a message - Zoom in to view individual microarray probes.

 

Peaks Track

The Peaks track displays the results of peak caller tasks. It displays a bar that spans genomic location of each peak call. If summits are identified by the peak caller, such as the MACS2 algorithm, then its genomic location is marked by a vertical line. The color marks either the pair being compared by the peak caller or, if present, the sample attribute associated with the sample.

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SubtitleTextPeaks track shown for two different pairs
AnchorNamePeaks track

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