Partek Flow Documentation

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After performing exploratory analyses such as PCA, UMAP and t-SNE is is helpful to visualize the results on a scatterplotscatter plot. This can help visually assess the source of variation affecting the results of an experiment, classify cells and select samples for downstream analysis. Here we have a PCA scatterplot scatter plot generated from the analysis of 12 samples from a scRNA sequencing study. The first three most informative PCs are plotted by default and the percentage of variation explained is stated next to each one of them.


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SubtitleTextExample of a 3D PCA scatterplot .
AnchorNameExample of a 3D PCA scatterplot

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The Configure > Style menu on the left can then be used to color the features in the scatterplot scatter plot based on an attribute (Figure 2). In this case, Figure 3 shows the cells being colored based on their cell-type.

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SubtitleTextCustomization menu.
AnchorNameCustomization menu

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SubtitleTextPCA scatterplot colored by cell-type.
AnchorNamePCA scatterplot colored by cell-type.

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SubtitleTextSplitting by an attribute can help better visualize their effect on the data.
AnchorNameSplitting by an attribute can help better visualize their effect on the data.


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Click the Save image button Image Added to save a PNG or SVG image to your computer. 

Click the Send to notebook button Image Added to send the image to a page in the Notebook. 


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