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Click the green plus () icon next to the Reference Files section header and choose Reference sequence from the Library type drop-down list in the Add reference sequence dialog (Figure 1). Alternatively, click the Add library file button and choose Reference sequence from the Library type drop-down list. If a cytoband file reference sequence is already associated with an assembly, Reference sequenceCytoband will be the only option available in the Add reference sequencegenome dialog and will not appear in a drop-down list (Figure 1).
Figure 1. Add reference sequence dialog. If the reference sequence and cytoband files have not been added yet, both options will appear in the Library type drop-down list (left). If one is missing, it will appear as the only option (right). For many model organisms, automatic downloads are available from the Partek repository.
If you are using an assembly supported by Partek (e.g. human), there are two radio button options: Download reference sequence and Import reference sequencecytoband and Create cytoband from 2bit (Figure 124). Select Download reference sequencecytoband and click Create to get the reference sequence cytoband file from the Partek repository. Alternatively, select Import reference sequenceCreate cytoband from 2bit and click Create to add build the cytoband file. If the reference sequence is missing, it will either be downloaded automatically or you will be asked to import it from another source (see Adding a reference sequence).
If you are using a custom assembly (e.g. for a non-model organism), only the Import reference sequence radio button will be Create cytoband from 2bit option is available (Figure 2).
Figure 2. For custom assemblies, automatic downloads are not available so the reference sequence has to be imported from another source
The Import reference sequence option allows you to add a reference sequence from the Partek Flow Server, My Computer or a URL download link (Figure 3).
Figure 3. A reference sequence can be added from multiple sources
To add a reference sequence from the Partek Flow server, select Partek Flow Server, click Browse and navigate to the directory storing the reference sequence file(s) (Figure 4). Only files with the correct file extensions (.fa, .2bit or any compressed format) will be displayed in the file browser. Select the checkbox next to the file name(s) you wish to import and click Continue (Figure 4). Click Finish to add the selected reference sequence file(s) (Figure 3).
Figure 4. Add a reference sequence file(s) from the Partek Flow Server
To upload a reference sequence from your local computer select My computer, click Choose file and navigate to the location of the file(s) on your local machine (Figure 5). It is not advisable to upload large files through the web browser, as this will likely result in an error (e.g. an upload timeout). Administrative users are able to set a limit on the upload file size via System preferences. The default file size limit is 500 MB. Please note that only one file can be uploaded at a time. Click Finish to add the selected reference sequence file(s) (Figure 5).
Figure 5. Upload a reference sequence file from your local computer through the browser
To download a reference sequence (e.g. from a public repository such as ENSEMBL), select URL and input the URL address (Figure 6). Make sure to include the full URL address, starting with the transfer protocol (e.g. http:// or ftp://) and ending with the file name. It is advisable to find the download link address, copy it to the clipboard and paste it into the Input URL box. Click Finish to add the selected reference sequence file(s) (Figure 6).
Figure 6. Download a reference sequence from the internet
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cytoband has to be created
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