Partek Flow Documentation

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To perform the task, select an aligned reads data node and click Convert alignments to unaligned reads task in the task menu (Figure 1). 

 

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SubtitleTextConvert alignments to unaligned reads
AnchorNameConvert alignments to unaligned reads

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A new data node will be generated containing FASTQ files (Figure 2) . Note that the files generated are compressed and the filenames are *.fastqfq.gz. 

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SubtitleTextUnaligned reads generated from aligned reads
AnchorNameUnaligned reads generated from aligned reads

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During the conversion, the BAM files are converted to FASTQ files.  If the BAM   The name of the BAM files will be based on the sample names in the Data tab. If the BAM file contains paired end reads, two FASTQ files will be generated for each BAM, and the files names will contain _1 and _2. An example  in Figure 3 shows 18 fastqFQ.gz GZ files from 9 .bam BAM files.

 

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SubtitleTextTask details of unaligned reads generated from aligned paired end samples
AnchorNameTask details of unaligned reads generated from aligned paired end samples

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Additional assistance

 


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