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• Select the Analysis tab
• Select 1-removeresult (batch-remove) in the spreadsheet tree
• Select Stat from the main toolbar
• Select ANOVA...
• Add Treatment, Time, and Batch factors to the ANOVA Factor(s) panel
• Add Treatment * Time interaction to the ANOVA Factor(s) panel
• Select ContratsContrasts...
• Select Treatment from the Select Factor/Interaction drop-down menu
• Select Yes for Data is already log transformed? 
  
• Set up contrasts of treatment vs. control for E2, E2+ICI, E2+Ral, and E2+TOT (Figure 7)
  
  

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