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• Select Create Marker List from the Analysis section of the Illumina BeadArray Methylation workflow
• Select the Advanced tab (Figure 1)
  
  

• Select Specify New Criteria to launch the Configure Criteria dialog
• Select female_only/anova-1way_autosomal (autosomal) as the source spreadsheet from the left-hand panelSelect from the Spreadsheet drop-down menu
• Select 12. Estimate(LCLs vs. B cells from the Contrast: find genes that change between two categories section of the ANOVA Streamlined tab of the List Manager dialog
• Set Fold change > to 20
• Set OR Fold change < to -20
• Set p-value with FDR to 0.005
• Set file name as LCLs vs. B cells
• Leave the rest of the option set to defaults (Figure 1)

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• cells) from the Column drop-down menu
• Set Include values to less than or equal to with -5 and greater than or equal to with 5 (Figure 2)
  
  

• Name this list; we have chosen differentially regulated
• Select OK

The criteria differentially regulated has been added to the Criteria list (Figure 3). 

• Select Specify New Criteria 
• Select female_only/anova-1way_autosomal (autosomal) from the Spreadsheet drop-down menu
• Select 6. p-value(LCLs vs. B cells) from the Column drop-down menu
• Set Include p-values to significant with FDR of at 0.005 (Figure 4)
  
  

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• Name this list; we have chosen <0.005
• Select OK

Both differentially regulated and <0.005 are listed in the Criteria panel. We can use the Combine criteria tools to generate a single combined filter. 

• Select Intersection (And) from Combine criteria to create a combined filter
• Name the new filter; we have chosen LCLs vs. B cells
• Select Save List (Figure 5)
  
  

• Select OK from the List Creator dialog to generate a list using the LCLs vs. B cells filter
• Select Close to exit the List Manager dialog

The new spreadsheet spreadsheet LCLs vs. B cells (LCLs vs. B cells) will open in the Analysis tab (Figure 2).   

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