Aligned reads data can be converted to unaligned reads in Partek^® Flow^®.  The task is available in the task menu when the user selects any aligned data node (Figure 1).  It , which can be from a result of an aligner in Partek Flow or data already aligned before importing into a projectimport.

Generating unaligned reads from aligned data gives you the flexibility to realign remap the reads using either a different aligner, a different set of alignment parameters, or a different genome reference. This is particularly useful in sequencing samples analyzing sequences from xenograft models where the same set of reads are can be aligned to two two different species. It is may also helpful be useful if the original unaligned FASTQ files are not as easily accessible to the user as the aligned BAM files.

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