Partek Flow Documentation

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To perform the task, select an aligned reads data node and click Convert alignments to unaligned reads task in the task menu (Figure 1). 

 

 

Numbered figure captions
SubtitleTextConvert alignments to unaligned reads
AnchorNameConvert alignments to unaligned reads

 

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During the conversion, the BAM files are converted to FASTQ files.  If the BAM file contains paired end reads, two FASTQ files will be generated for each BAM, and the files names will contain _1 and _2. In Figure 3, the example An example  in figure 3 shows 18 fastq.gz files from 9 .bam files.

 

Numbered figure captions
SubtitleTextTask details of unaligned reads generated from aligned paired end samples
AnchorNameTask details of unaligned reads generated from aligned paired end samples

 

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