Partek Flow Documentation

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  • This results in two starting nodes, one for single cell counts and one for single cell V(D)J as shown below. Note that once subsequent tasks are performed on a node, no more data can be imported into this project. The single cell counts node can be processed as usual; for help information related to this please see the tutorial for Analyzing Single Cell RNA-Seq Data.

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References

  1. Tonegawa, S. Somatic generation of antibody diversity. Nature 302,575–581 (1983). https://doi.org/10.1038/302575a0
  2. https://support.10xgenomics.com/single-cell-vdj/software/pipelines/latest/output/annotation#contig-annotation 
  3. https://support.10xgenomics.com/single-cell-vdj/software/overview/welcome 
  4. https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/7.0/advanced/h5_matrices
  5. https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/7.0/output/matrices
  6. https://support.10xgenomics.com/single-cell-vdj/software/pipelines/latest/using/vdj 
  7. https://support.10xgenomics.com/single-cell-vdj/software/pipelines/latest/using/multi