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- Select the Trimmed reads data node
- Select Aligners from the task menu to display available aligners (Figure 1)
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Partek Flow offers a variety of different aligners. Mouse over any option for a short description. For this tutorial, we will use STAR, a fast and accurate aligner commonly used for RNA-Seq data. For more information about STAR and the other aligners, please consult the Aligners user guide.
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- Select Homo sapiens (human) - hg19_chr22 from the Assembly drop-down menu
- Select Whole genome from the Aligner index drop-down menumenu
- Select Finish (Figure 2)
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Alignment is a resource-intensive task and may take a while to complete, even mapping only to chromosome 22. Nodes that have been queued, but not completed are shown in a ligher color than completed tasks (Figure 3).
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- Mouse over the Align reads task node to view the estimated end time and progress percentage (Figure )
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