Partek Flow Documentation

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  • Select the Trimmed reads data node
  • Select Aligners from the task menu to display available aligners (Figure 1)

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SubtitleTextA variety of different aligners are available in Partek Flow. We will use STAR, a popular choice for aligning RNA-Seq data, in this tutorial
AnchorNameInvoking aligner

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Partek Flow offers a variety of different aligners. Mouse over any option for a short description. For this tutorial, we will use STAR, a fast and accurate aligner commonly used for RNA-Seq data. For more information about STAR and the other aligners, please consult the Aligners user guide. 

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  • Select Homo sapiens (human) - hg19_chr22 from the Assembly drop-down menu
  • Select Whole genome from the Aligner index drop-down menumenu 
  • Select Finish (Figure 2)

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SubtitleTextConfiguring the STAR aligner
AnchorNameConfiguring STAR aligner

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Alignment is a resource-intensive task and may take a while to complete, even mapping only to chromosome 22. Nodes that have been queued, but not completed are shown in a ligher color than completed tasks (Figure 3).

 

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SubtitleTextQueued tasks and their output data nodes are shown in a lighter color
AnchorNameQueued tasks

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  • Mouse over the Align reads task node to view the estimated end time and progress percentage (Figure )

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  • 4)

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SubtitleTextProgress and time to finish estimates are available for any queued task
AnchorNameViewing progress

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The Align reads task generates an Aligned reads data node once complete. If you would like, you can return to Partek Flow when the alignment task has completed, or you can continue building the pipeline while the results of alignment are pending. Additional tasks can be added to the pipeline and queued before the current task has completed.

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