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Before performing pathway enrichment analysis, we need to create a gene list from the ANOVA results. 

Creating a list of significant genes

• Select Gene Expression from the workflows drop-down menu
• Select the ANOVA results spreadsheet
• Select Create Gene List from the Analysis section of the Gene Expression workflow
• Select Brain vs. Heart from the List Manager dialog (Figure 1)
• Select Create 
  
  

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• Select Close to exit the List Manager dialog

Performing pathway enrichment analysis 

• Select the new gene list, Brain vs. Heart
• Select Pathway Analysis from the Biological Interpretation section of the Gene Expression workflow
• Select Next > to continue with Pathway Enrichment

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