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The significant CpG loci detected in the previous step actually form a methylation signature that differentiates , which we shall now visualise by a heat map.
- Select the (LCLs vs. B cells) cells spreadsheet in the spreadsheet pane on the left
- Select Cluster Based on Significant Genes from the Visualization panel of the Illumina BeadArray Methylation workflow
- Select Hierarchical Clustering for Specify Method (Figure 1)
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- Select OK
- Verify that (CpG of interest) that LCLs vs. B cells is selected in the drop-down menu
- Select Standardize for Expression normalization (Figure 2)
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