Webinar April 1 and 2
Tissue Transcriptomics Data Analysis in Partek Flow: Data Import, Analysis, and Visualization Registration and Details
Partek Pathway is a separate program from Partek Genomics Suite with a distinct user interface (Figure 1).
Figure 1. Partek Pathway
The Project Elements panel (Figure 2) displays the selected pathway, the original gene list, the Pathway Enrichment spreadsheet, and the library references that were used for the pathway analysis. The Project Elements panel is used to navigate between open pathway diagrams and spreadsheets.
The Brain vs. Heart gene list we created earlier will open (Figure 3). The spreadsheet can be sorted by any column by left-clicking a column header; the first click will sort by ascending values, the second click will switch to descending values.
The Pathway-Enrichment.txt spreadsheet will open (Figure 4). This spreadsheet has the same contents as the Pathway-Enrichment.txt spreadsheet in Partek Genomics Suite. Selecting any of the pathway names will open its pathway diagram. The spreadsheet can be sorted by any column.
The GABAergic synapse pathway diagram will open. Genes in the pathway are shown as boxes. The color of the boxes is set by the Configuration panel (Figure 5).
Any numerical column from the source gene list can be used to color the gene boxes. While significant p-values indicate a difference between the categories, they give no information about upregulation or downregulation of the pathway. We can overlay fold-change information on the pathway diagram.
The pathway diagram now shows fold change for each gene in the pathway included in the gene list (Figure 6). Genes not in the gene list remain black.
The colors and range of can be changed using the Color By panel.
We can see that all the colored genes in the GABAergic synapse pathway are yellow (Figure 7), indicating that they are up-regulated.
We can select a gene to learn more about it.
Options available include:
Look up on KEGG - opens the KEGG page for the pathway on GenomeNet (genome.jp) in your web browser
Ensembl - under External Links, opens the page for the selected Ensembl ID on ensemble.org
UniProt - under External Links, opens the page for the selected UniProt ID on uniprot.org
Jump to ___ on "___" - opens the source gene list in Partek Pathway to the row of the selected gene
Selecting () activates Navigation Mode. This enables navigation on large pathway diagrams by left-clicking and dragging to move the view.
The pathway database can be searched for genes of interest using the Search panel.
Pathways containing NSF appear on the right-hand side in the Search Results section in alphabetical order (Figure 9).
If multiple species or libraries have been loaded, the Filter Options section on the left-hand side can be used to choose which species and libraries to search.
The selected pathway, Synaptic vesicle cycle, will open in the Pathway Diagram panel (Figure 10).
On the right-hand side of the Partek Pathway window, we see the Pathway Detail panel (Figure 11).
Selecting a gene in the list will highlight it in the pathway diagram (Figure 12).
Another way to select and view a pathway is browsing the Pathway Libraries.
The Pathway Libraries dialog will open (Figure 13).
In the upper section of the dialog, you can view available KEGG libraries and download them by selecting the Download Library link. Selecting a pathway opens it in the lower section of the dialog.
In the lower section of the dialog, you can view a list of all the pathways in the selected pathway library. You can also open any pathway from the selected library in the Pathway Diagram panel.
We can use the Project Elements panel to close an open pathway diagram or list.
The Search panel and Pathway Libraries can also be used to open pathway diagrams for pathways without any open gene or pathway lists.
If you need additional assistance, please visit our support page to submit a help ticket or find phone numbers for regional support.