Performing tasks in the Analyses tab
A context-sensitive menu will appear on the right-hand side of the pipeline. This toolbox includes tasks that can be performed on the selected data node.
An important step in analyzing single cell RNA-Seq data is to filter out low-quality cells. A few examples of low-quality cells are doublets, cells damaged during cell isolation, or cells with too few counts to be analyzed. Click here for more information on Single cell QA/QC. We will not perform Single cell QA/QC in this tutorial.
- Click the Filtering drop-down in the toolbox
- Click the Filter Features task
- Choose Noise reduction
- Exclude features where value <= 0.0 in at least 99.0% of the cells
- Click Finish
A task node, Filtered counts, is produced. Initially, the node will be semi-transparent to indicate that it has been queued, but not completed. A progress bar will appear on the Filter features task node to indicate that the task is running.
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