Illumina’s MethylationEPIC array interrogates the methylation status of over 850,000 cytosines in the human genome. Because the MethylationEPIC array is closely related to the Infinium HumanMethylation450 BeadChip, the steps presented in this document can be applied to either platform.
This tutorial illustrates how to:
Note: the workflow described below is enabled in Partek Genomics Suite version 7.0 software. Please fill out the form at www.partek.com/PartekSupport to request this version or use the Help > Check for Updates command to check whether you have the latest released version. The screenshots shown within this tutorial may vary across platforms and across different versions of Partek Genomics Suite.
Due to annotation changes, the results presented here may slightly differ over the time.
Description of the Data Set
The data set accompanying this document consists of sixteen human samples processed by Illumina MethylationEPIC arrays. The data set is taken from a study of DNA methylation in human B cells and B cells infected with Epstein-Barr virus (EBV).
Infecting B cells with EBV in vitro transforms them, making them capable of indefinite growth in vitro. These immortalized cell lines are referred to as lymphoblastoid cell lines (LCLs). LCLs behave similarly to activated B cells, making them useful for expanding T cells in vitro. Because EBV is a carcinogen and immortalized cell growth is a hallmark of cancer, examining the effects of EBV transformation on B cell DNA methylation might shed light on the roles of DNA methylation in tumor development.
The data files can be downloaded from Gene Expression Omnibus using accession number GSE93373. To follow this tutorial, download the 32 .idat files associated with GSE93373 (note that two .idat files are generated for each array) and unzip them on your local computer using 7-zip, WinRAR, or a similar program. The .idat files can be downloaded individually by selecting the links for each sample or altogether in one zipped folder by selecting the GSE93373_RAW.tar file at the bottom of the page.
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