PGS Documentation

Page tree
Skip to end of metadata
Go to start of metadata

You are viewing an old version of this page. View the current version.

Compare with Current View Page History

« Previous Version 2 Next »

We can check the quality of the samples using Partek Genomics Suite before analyzing the data. 

Strand cross-correlation

Text goes here. 

Checking the distribution of reads

BAM files contain both aligned and unaligned reads. The spreadsheet created during import shows the number of reads that were aligned to the reference genome. A large number of unaligned reads may be the result of poor quality sequencing data or alignment problems. It may also be useful to know how many reads map to more than one location in the genome if the options used during alignment supported multiple-mapped reads. 

  • Select Alignments per read form the QA/QC section of the ChIP-Seq workflow (Figure 1)

A new spreadsheet named Alignment_Counts will be generated (Figure 2). 

 

 

Additional Assistance

If you need additional assistance, please visit our support page to submit a help ticket or find phone numbers for regional support.

Your Rating: Results: 1 Star2 Star3 Star4 Star5 Star 1 rates

  • No labels