Partek Flow Documentation

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SubtitleTextClick and drag the Scree plot to replace the PCA plot on the canvas
AnchorNameReplace PCA with Scree plot

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  • Select PCA as data for the new Scree plot (Figure 5)

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SubtitleTextExample of how the pipeline might look if you split the merged counts and perform exploratory analysis for protein and gene expression data separately
AnchorNameSplit merged counts for exploratory analysis

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You can then use the Data viewer to bring together multiple plots for comparison (Figure 14).

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SubtitleTextComparison of 2D UMAP plots for the same cells clustered on protein, mRNA and merged data. All cells are coloured based on their expression of the CD3D gene (in blue). Note, the plots in this figure may differ from the default UMAP plots because these are 2D plots. Default UMAP plots re in 3D.
AnchorNameUMAP plot comparison

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