Partek Flow Documentation

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The library file management tool in Partek® Flow® provides an easy way to create, process and manage reference sequences, cytoband files, annotation models, aligner indexes, gene sets, variant databases and microarray probe sequence files.   

This user guide will cover the following topics:


For a quick start, you can watch the short video below.

Quick Video Demo of Library File Management

The short clip below (video only, no audio) shows how to quickly setup some library files for the hg19 human reference genome assembly. The process can be adapted to the different kinds of file types.

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Library File Management settings

To review and edit library file management settings, you must be logged into Partek Flow as a user with administrator privileges. Click on the avatar in the top right corner and choose Settings. Then click System preferences on the left.

You can review the current library file directory location and privilege settings that determine whether non-administrative users can add or remove library files (Figure 1).

  

Selecting an Assembly

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SubtitleTextSystem preferences page showing library file directory location and privilege settings. Settings can be changed by clicking on Edit system preferences at the bottom
AnchorNamesys-preferences

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To change where the library files are stored, click Image Added Edit file system and storage and click Browse to point to another directory (Figure 2).

 

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SubtitleTextChanging where the library files are stored
AnchorNamelibrary-file-dir

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If you only want administrative users to manage library files, click Image Added Edit user quotas and limitations and select the checkbox shown in Figure 3. Non Administrative users will still be able to view the library files. Unselecting the checkbox will allow any user to manage library files, but only administrative users can remove entire assemblies. The assemblies and library files created by non administrative users will be available for all Partek Flow users.

 

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SubtitleTextChange whether administrative or non administrative users can manage library files.
AnchorNameadmis-manage-library-files

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Library File Management Page

To access the library file management page click the avatar in the top right corner and choose Settings. Then click Library file management on the left.

The library file management page has two tabs - Genomic library files and Microarray library files. This section of the user guide will focus on the Genomic library files tab, which is relevant for next-generation sequencing analysis (Figure 4).

 

 

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SubtitleTextGenomic library files page
AnchorNamegenomic-library-files-page

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The gray arrows (Image Added/Image Added) expand/collapse each section. Associated library files are shown in a table in each section. The view file (Image Added) icon in the Actions column of each table displays additional library file details. The red cross (Image Added) dissociates a library file. The hourglass (Image Added) icon indicates a library file is being created

Selecting an Assembly

Choosing an Assembly from the drop-down list at the top will display the associated library files in the pane below. Assemblies are named by the species and the build version (e.g. Homo sapiens (human) - hg19). Different build versions for the same species are regarded as separate assemblies (Figure 5). Administrative users can delete a selected assembly by clicking the red cross (Image Added).

 

 

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SubtitleTextHuman assembly versions hg17, hg18 and hg19 will appear as separate assemblies in the drop-down list.
AnchorNamehuman-assembly-versions

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