Partek Flow Documentation

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SubtitleTextThe figure legends show 3696/4352 cells with total count <12,000, number of detected features 600 - 2500, and % mitochondrial count <5% are selected in all three plots. Selected cells are blue, filters are additive and deselected cells are dimmed.
AnchorNameSingle cell QAQC with selection criteria applied

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To filter the high-quality cells, click the  icon in Filter in the top right of Select & Filter, and click Apply observation filter... (Figure 9).

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SubtitleTextAfter the Apply filter button is selected, you will be presented with a preview of your pipeline. You need to select the appropriate data node to apply the filtering to
AnchorNameSelect input data node for filtering

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A new data node, Filtered counts, will be generated under the Analyses tab (Figure 11).

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SubtitleTextFilter cells task runs from the Single cell QA/QC report
AnchorNameOutput of Single cell QA/QC

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Double click the Filtered counts data node to view the task report. The report includes a summary of the count distribution across all features for each sample; a detailed breakdown of the number of cells included in the filter for each sample; and the minimum and maximum values for each quality metric (expressed genes, total counts, etc) across the included cells for each sample (Figure 12).

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