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Select the source of annotation file and click Next. You must then specify the type of annotation file by choosing an option from the Annotation data type drop-down list (Figure 35). Choose mRNA for whole transcriptome annotations, where both gene and transcript level information is are present. Choose microRNA for precursor or mature microRNA transcript annotations. Choose Amplicon for targeted amplicon sequencing annotations. Choose Other for annotation files that do not fall into the other three categories (e.g. lncRNA).
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For text annotations with both gene- and transcript-level information select mRNA from the drop-down menu. You can manually specify which column corresponds to the Transcript ID or Gene ID by selecting the corresponding radio button next to the column with that information.
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Adding Aligner Indexes Based on an Annotation Model
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