Flow Documentation

Partek Flow Documentation

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Numbered figure captions
SubtitleTextComparison of expected fold change (log2) vs. observed fold change (log2) in ERCC Mix A vs. Mix B, for the four groups of transcripts with the known fold change values. Each dot is an ERCC control. r^2 = 0.87, regression y = 0.96 * x + 0.09
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To test for possible biases in abundance estimates, we combined the approaches of Li et al. (9) and Jiang et al. (12). That amounted to regressing the estimated abundance not only on the expected abundance, but also on the transcript length, GC content, and the expected fold change, subjecting all the variables to log2 transformation.

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