Comparison of expected number of molecules of ERCC Mix B and the estimated number of transcript molecules (log2 of RPKM values), obtained by Partek’s modification of expectation-maximization algorithm. Each dot is an ERCC control. R2 r^2 = 0.97, regression y = 0.98 * x - 74.06
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Comparison of expected fold change (log2) vs. observed fold change (log2) in ERCC Mix A vs. Mix B, for the four groups of transcripts with the known fold change values. Each dot is an ERCC control. R2 r^2 = 0.87, regression y = 0.96 * x + 0.09