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Once the analysis is complete, open the resulting Feature list data node. Navigate to the Gene list section of the page and click on the volcano plot icon, located on the right left of the table header (Figure 1).  

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Each point on the plot represents the statistical result for a single feature (e.g. gene, transcript etc.). The black vertical and horizontal lines represent threshold of fold change and p-value respectively.  By By default the two vertical lines represent fold changes of -2 and +2, the horizontal line represents significant p-value of 0.05. The number of features Features that are up/down-regulated by at least 2 fold and have a p-value less than 0.05 are at the upper-right and lowerupper-left corner of the plot, they are highlighted in different colors. By default, significantly up-regulated features are in red, significantly down-regulated features are in green. The blue, and the remaining features are in grey or black (Figure 2). The plot header is derived from the name of the contrast.

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The Axes and Statistics menus allow to customize which data sources will be used for the axes as well as annotations, limits and significance thresholds for the plot (Figure 3).

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The colors can also be customised using the Style menu (Figure 4).

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You can also highlight and label any of the genes in the plot (Figure 5).

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Click the Save image button Image Removed the Export image button on the left panel to save a PNG, SVG, or SVG PDF image to your computer. 

Click the Send to notebook button Image Removed to To notebook button on the left panel to send the image to a page in the Notebook. 

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