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exclude | Additional Assistance |
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Create a new Project
Let's start by creating a new project.
- On the Home page, click New project (Figure 1)
- Give the project a name
- Click Create project
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SubtitleText | Create a new project and give it a meaningful name (e.g. CITE-Seq tutorial) |
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AnchorName | Creating a new project |
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Import data
- In the Analyses tab, click Add data
- Click 10x Genomics Cell Ranger counts h5 (Figure 2)
- Choose the filtered HDF5 file for the MALT sample produced by Cell Ranger
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SubtitleText | Import options for CITE-Seq tutorial data |
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AnchorName | Import options for CITE-Seq tutorial data |
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Move the .h5 file to where Partek Flow is installed using , then browse to its location.
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SubtitleText | Import options for CITE-Seq tutorial data |
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AnchorName | Import options for CITE-Seq tutorial data |
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Note that Partek Flow also supports the feature-barcode matrix output (barcodes.tsv, features.tsv, matrix.mtx) from Cell Ranger. The import steps for a feature-barcode matrix are identical to this tutorial.
- Click Next
- Name the sample MALT (the default is the file name)
- Specify the annotation used for the gene expression data (here, we choose Homo sapiens (human) - hg38 and Ensembl Transcripts release 109). If Ensembl 109 is not available from the drop-down list, choose Add annotation and download it.
- Check Features with non-zero values across all samples in the Report section
- Click Finish (Figure 3)
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SubtitleText | File format options for MALT data set |
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AnchorName | CITE-Seq file format options |
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A Single cell counts data node will be created under the Analyses tab after the file has been imported. We can move on to processing the data.