To run Hashtag demultiplexing, your data must meet the following criteria:
- Cell Hashing features must have a different data type than your gene expression or protein expression data
- Cell Hashing data should be normalized with CLRData node contains number of features less than number of observations
- Data node must be output from normalization task (recommended normalization method for hashtag is CLR)
If you are processing your FASTQ files in Partek Flow, be sure to specify a different Data type for your Cell Hashing FASTQ files on import than the FASTQ files for your gene expression and any other antibody data.