Partek Flow Documentation

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A task node, Single cell QA/QC, is produced. Initially, the node will be semi-transparent to indicate that it has been queued, but not completed. A progress bar will appear on the Single cell QA/QC task node to indicate that the task is running (Figure 1).

 

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SubtitleTextAnalyses tab
AnchorNameAnalyses tab

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  • Click the Single cell QA/QC node once it finishes running
  • Click Task report in the task menu 

The Single cell QA/QC report includes interactive violin plots showing the value of every cell in the project on several quality measures (Figure 12).

 

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SubtitleTextSingle cell QA/QC plot
AnchorNameSingle cell QA/QC

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  • Click the Noise reduction filter check box 
  • Set the Noise reduction filter to Exclude features where value == 0 in 100% of cells using the drop-down menus and text boxes
  • Click Finish to apply the filter (Figure 3)

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SubtitleTextFilter features
AnchorNameFilter features

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This produces a Filtered counts data node. This will be the starting point for the next stage of analysis - identifying cell types in the data using the interactive t-SNE plot. 

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  • Click Finish to apply the normalization (Figure 4)

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SubtitleTextNormalization
AnchorNameNormalization

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A new Normalized counts data node will be produced.

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