Partek Flow Documentation

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The Trim bases task will generate a new data node, Trimmed reads. While tasks have been queued or are in progress they have a lighter color. Any output nodes that the task will generate, such as the  (Figure 3). We can view the task report for Trim bases by double-clicking either the Trim bases task node or the Trimmed reads data node from the Trim bases task, are also displayed in a lighter color until the task completes. Once the task begins running, a progress bar is displayed on the task node (Figure 3).or choosing Task report from the task menu. 

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SubtitleTextTask and Data nodes that have been queued or are in progress are shown in a lighter color than completed tasks.
AnchorNameQueued task node

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We can view the task report for Trim bases by selecting either the Trim bases task node or the Trimmed reads data node and choosing Task report from the task menu. 

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  • Double-click the Trimmed reads data node Select Task report from to open the task menureport

The report shows the percentage of trimmed reads and reads removed in a spreadsheet (Figure 4) and a graph two graphs (Figure 54).  

 

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SubtitleTextSpreadsheet showing results Results of the Trim bases task
AnchorNameTrim bases report spreadsheet

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SubtitleTextBar chart showing results of Trim bases task
AnchorNameTrimming reads graph

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The average quality score for each base call at each position for each sample is also shown as a graph (Figure 6).

 

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SubtitleTextUpdated base call quality graph after Trim bases task
AnchorNameUpdated base quality

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The results are fairly consistent across samples with ~65% of reads untrimmed, ~30% trimmed, and ~5% removed for each. The average quality score for each sample is increased with higher average quality scores at the 3' ends. 

 

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