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SubtitleText | Invoking Pool cells |
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AnchorName | Invoking pool cells |
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Options for Pool cells are Sum, Maximum, Mean, and Median. Expression values for cells from the same sample with the same cell type classification will be merged using the chosen pooling method (Figure 2). Sum is selected by default. After choosing a pooling method, select Finish to run the Pool cells task.
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SubtitleText | Choosing the pooling method |
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AnchorName | Choosing pooling method |
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Ouput of Pool cells
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SubtitleText | Output of Pool cells. This data set had 3 cell types, Malignant, Microglia/macrophages, and Oligodendrocytes. Pool cells has creates a counts data node for each cell type. |
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AnchorName | output of pool cells |
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Each counts data node is equivalent to simulated bulk RNA-Seq counts data for a cell type. The same tasks that can be performed on bulk RNA-Seq counts data can be performed on Pool cells output data nodes, including normalization, filtering, PCA, and differential expression analysis.
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SubtitleText | Pool cells counts data can be downloaded as a tab-delimited text file. The downloaded text file can be opened in any spreadsheet viewing program or text editor. |
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AnchorName | Pool cells file |
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