We can use Attributes are used to describe your samples. Examples of sample attributes include treatment group, age, sex, and time point. Attributes can be added manually or using a tab-delimited text file to add sample attributes. The text file should have with samples on rows and attributes on columns with where the first column containing contains the sample IDs. In this tutorial, we will add one attribute, Treatment, manually.

• Select the Data tab
• Select Assign sample attributes from a file Manage attributes (Figure 1)
  
  

...

• Select Add new attribute (Figure 2)
  
  

• Select Next
• Select the Import checkbox for Treatment Set Treatment as Name
• Select Categorical as Attribute type 
• Select Project-specific for Visibility 
• Select Add (Figure 3)
  
  

...

• Select Import
  

• Name the first New category 0uM
• Select Add
• Name the second New category 5uM
• Select Add
• Name the third New category 10uM
• Select Add
  
• Select Close to finish creating the attribute (Figure 4)
  
  

• Select Back to sample management table 

The data table now includes an Attribute column for Treatment (Figure 5). Next, we need to assign samples attribute categories for Treatment.

• Select Edit attributes

The option to edit the Treatment field for each sample will appear as a drop-down menu (Figure 6).

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• Select the Treatment text box for a sample to bring up a drop-down menu with the Treatment attribute categories (0uM, 5uM, 10uM)
• Use the drop-down menus to add a treatment group for each sample

The first three samples (SRR592573-5) should be 0uM, the next three samples (SRR592576-8) should be 5uM, and the final three samples (SRR592579-81) should be 10uM (Figure 7).

The data table will now show each a Treatment attribute for each sample.