Partek Flow Documentation

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  • Double click the Feature list data node to open the task report (Figure)

The task report spreadsheet will open showing genes on rows and the results of the GSA on columns (Figure 1).

 

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SubtitleTextViewing the GSA task report spreadsheet
AnchorNameTask report spreadsheet

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To get a sense of what filtering thresholds to set, we can view a volcano plot for a comparison. 

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A volcano plot will open showing p-value on the y-axis and fold-change on the x-axis (Figure 2). 

 

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SubtitleTextViewing GSA results with a volcano plot
AnchorNameVolcano plot

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Thresholds for the cutoff lines are set using the text boxes on the left-hand side. The default thresholds are and 0.05

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Note that the number of genes that pass the filter is listed at the top of the filter menu next to Results: and will update to reflect any changes to the filter. Here, 40 21 genes pass the filter (Figure).

 

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SubtitleTextApplying filters to the GSA results spreadsheet
AnchorNameFiltering a gene list

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  • Select  to create a data node with only the genes that pass the filter

This creates a Filtered gene analysis task node and a Feature list data node (Figure).

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SubtitleTextFiltered gene analysis task node and Feature list data node
AnchorNameFeature list data and task nodes

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