Join us for a webinar: The complexities of spatial multiomics unraveled
May 2

Partek Flow Documentation

Page tree

Versions Compared

Key

  • This line was added.
  • This line was removed.
  • Formatting was changed.

...

Numbered figure captions
SubtitleTextInvoking the Trim bases task
AnchorNameTrim bases

By default, Trim bases removes bases removes bases starting at the 3' end and continuing until it finds a base pair call with a Phred score of equal to or greater than 20. Hover over the Image Removed for additional information on any task option 35 (Figure 2). 

  • Click Finish to run Trim bases with default settings

...

Numbered figure captions
SubtitleTextViewing additional information about the Quality score trimming methodConfiguring Trim bases
AnchorNameConfiguring Trim bases

...

Numbered figure captions
SubtitleTextA Task and a Data node are created from the Trim bases task. Task and Data nodes that have been queued or are in progress are shown in a lighter color than completed tasks.
AnchorNameQueued task node

 

...

The results are fairly consistent across samples with ~50% ~2% of reads untrimmed, ~45% ~86% trimmed, and ~5% ~12% removed for each. The average quality score for each sample is increased with higher average quality scores at the 3' ends. 

...