In contrast to the dot plot which shows one probe(set)/gene per plot, the profile plot is used to visualize how the intensity values from multiple genes compare across all samples.

We will invoke a Profile Plot from a gene list child spreadsheet with genes on rows. 

• Select the rows to be visualized 
• Right-click on a row header of one of the selected rows
• Select Profile Plot (Orig. Data) from the pop-up menu (Figure 1)
  
  

The profile plot will be displayed in a new tab (Figure 2). Lines are probe(sets)/genes and columns are rows/samples from the parent spreadsheet. 

A basic profile plot will likely need customization. The plot configuration, properties, and control options are the same as in Dot Plot. We will illustrate a few modifications here. 

We can change the row labels to show each sample ID. 

• Select () 
• Select the Axes tab
• Set Grid to 1
• Select Rotate X-Axis Labels and set to 90 degrees (rotates counter-clockwise) 
• Set Label Format to Column and select 5. Subject

We can add symbols to show which group each sample belongs to.

• From the Shape by drop-down menu, select 3.Type 
  
• Select OK
  

Symbols have now been added to each profile line plot (Figure 3).