Analysis of differentially methylated loci in humans and mice typically includes exclusion of the probes on X and Y chromosomes because of the difficulties caused by the inactivation of one X chromosome in female samples. To filter out probes from the X and Y chromosomes, we first need to annotate the ANOVA spreadsheet with chromosome locations.

• Right-click on the Gene Symbol column 
• Select Insert Annotation (Figure 1) 
  
  

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