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• Select the annotation file; we have selected hg19_refseq_14_01_03_v2.pannot from the C:/Microarry Libraries folder
• Delete or rearrange the columns as necessary; we have placed the column with identifiers that correspond to our gene list first
• Select (Image Removed) Select File then Save As Text File... to save the annotation file; we have named it Annotation File (Figure 2)
  
  

• Select () to close the annotation file

Now we can add the annotation file to our imported gene list. 

• Right click 1 (Gene Listgene_list.txt) in the spreadsheet tree
• Select Properties from the pop-up menu

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• Select Browse under Annotation File
• Choose the annotation file; we have chosen Annotation File.txt txt (FIgure 

If this is the first time you have used an annotation, the Configure Annotation dialog will launch. This is used to choose the columns with the chromosome number and position information for each feature. Our example annotation file has chromosome, start, and stop in separate columns. 

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