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  • Select Create Marker List from the Analysis section of the Illumina BeadArray Methylation workflow
  • Select 1/anova-1way_autosomal_only (ANOVA-1way_autosomal_only) as the source spreadsheet from the left-hand panel
  • Select Naive shPOU5F1 and Naive shNANOG and Primed shCTRL vs. Naive shCTRL from the Contrast: find genes that change between two categories section of the ANOVA Streamlined tab of the List Manager dialog
  • Set Fold change > to 3
  • Set OR Fold change < to -3
  • Set file name as CpG of interest
  • Leave the rest of the option set to defaults (Figure 1)

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  • Select Create
  • Select Close to exit the List Manager dialog

The new spreadsheet Naive_shPOU5F1_and Naive_shNANOG_and_Primed_shCTRL_vs_Naive_shCTRL (CpG of interest) will open in the Analysis tab (Figure 2). 

 

Numbered figure captions
SubtitleTextFiltered marker list
AnchorNameFiltered Marker List

Image Added

You may want to save the project before proceeding to the next section of the tutorial.

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