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The Create categorical attribute dialog allows us to create groups for a categorical attribute. By default, two groups are created, but additional groups can be added. 

• Set Attribute name: to StateRename the groups Primed and NaiveExperimental Group
  
• Select New Group twice to add two additional groups
• Rename the groups Primed+shCTRL, Naive+shCTRL, Naive+shPOU5F1, and Naive+shNANOG
• Drag and drop the samples from the Unassigned list to their groups as listed in the table below

There should now be two four groups with two samples in each group (Figure 4).

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• Select OK
• Select Yes from the Add another categorical attribute dialog
• Set Attribute name: to shRNA treatment
• Select New Group to add an additional group
  
• Rename the three groups Control, shPOU5F1, and shNANOG
• Drag and drop the samples from the Unassigned list to their groups as listed in the table below

There should now be three groups with samples in each group (Figure 5).

• Select OK
• Select No from the Add another categorical attribute dialog
• Select Yes to save the spreadsheet

Two A new column as s been added to spreadsheet 1 (Differential Methylation Analysis) with the state and shRNA treatment of each sample (Figure 6). 

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