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The Create categorical attribute dialog (Figure 3) allows us to create groups for a categorical attribute. By default, two groups are created, but additional groups can be added. 

 

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SubtitleTextCreate Categorical Attribute is used to define new groups and assign samples to them. The name of new column (attribute) is specified in the Attribute name field, group labels (attribute levels) are specified in the Group Name fields. To assign samples to groups, use drag and drop (to select more than one, use Ctrl or Shift buttons)
AnchorNameassigning samples to groups

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  • Set Attribute name: to State
  • Rename the groups Primed and Naive
  • Drag and drop the samples from the Unassigned list to their groups as listed in the table below

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There should now be two groups with samples in each group (Figure 4).

 

 

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SubtitleTextAdding State attribute as a categorical group
AnchorNameState attribute

 

  • Select OK
  • Select Yes from the Add another categorical attribute dialog
  • Set Attribute name: to shRNA treatment
  • Select New Group to add an additional group
  • Rename the three groups Control, shPOU5F1, and shNANOG
  • Drag and drop the samples from the Unassigned list to their groups as listed in the table below

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