...

• Select GO Enrichment in the the Gene Set Analysis dialog and then select Next dialog (Figure 2)
• Select Next > 
  
  

• Choose Select the spreadsheet 1/Diff_Exp_and_Alt_Splice (Diff Exp and Alt Splice.txt) and select Next

...

•  from the drop-down menu (Figure 3)
• Select Next >
   
  

• Select Use Fisher's Exact test 
• Select Invoke gene ontology browser on the result
• Set Restrict analysis to functional groups with more than _ genes to 2 (Figure 4)
• Select Next >
  
  

• Select Default mapping file (Figure 5)
• Select Next >