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Hierarchical Clustering

The gene list in spreadsheet Down_Syndrome_vs_Normal (A) can be used for hierarchical clustering to visualize patterns in the data.

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For more information on the methods used for clustering, you can refer to Chapter 8: Hierarchical & Partitioning Clustering in Help > User’s Manual. For a tutorial on configuring the clustering plot, please refer to the user guide that can be downloaded from: here or from Help >On-line Tutorials > User Guides.

Adding Gene Annotation

During data importation, the GeneChip annotation file was linked to the imported data. This linked annotation information can be added as new columns to the ANOVA or gene list spreadsheets. For example, we can add additional annotation to the gene list we created from the ANOVA results as follows:

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SubtitleTextAdding Chromosomal Location annotation
AnchorNameAdd Rows/Columns to Spreadsheet

Interestingly, of the 23 genes of the Down_Syndrome_vs_Normal (A) spreadsheet, 20 genes are located on chromosome 21.  To This suggests that the gene expression changes associated with Down syndrome observed in this study are primarily located on chromosome 21. 

To save changes to the spreadsheet, select the Save Active Spreadsheet icon ().

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SubtitleTextDot Plot showing DSCR3 expression levels for each sample
AnchorNameDot Plot

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In the plot, each dot is a sample of the original data. The Y-axis represents the log2 normalized intensity of the gene and the X-axis represents the different types of samples. The median expression of each group is different from each other in this example. The median of the Down syndrome samples is ~6.3, but the median of the normal samples is ~6.0. The line inside the Box & Whiskers represents the median of the samples in a group. Placing the mouse cursor over a Box & Whiskers plot will show its median and range. 

 

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