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Therefore, the differential methylation analysis of the tutorial data will be performed on the M-values; select Convert Beta Value to M Value from the workflow. Note that the original data (β-values) will be overwritten.
Before proceeding to exploratory data analysis, the study samples need to be organized in four groups, two biological replicates each. In the Import section of the workflow, select Add Sample Attributes and, in the next dialog (Figure 2), Add a Categorical Attribute (and then OK).
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