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SubtitleText | Configuring criteria for transcripts with a p-value < 0.05 |
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AnchorName | Configure Criterea |
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- In the Configure Criteria dialog box (Figure 2), provide a name for the list (Diff Exp)
- Select 1/transcripts (RNA-Seq_results.transcripts) from theSpreadsheet drop-down menu
- Select 8. p-value(DiffExp) from the Column drop-down menu
- Set Include p-values to significant with FDR with a value of 0.05
A list of 3038,305 285 transcripts that pass this criteria will be generated according to the # pass score on the right-hand side of the dialog. If the settings are changed, this number will automatically update.
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SubtitleText | Configuring criteria for a list of alternatively spliced genes |
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AnchorName | Configuring Gene List another |
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- Select OK to generate Alt Splice
- Select both lists in the right-hand panel under the Criteria panel while holding the Ctrl key on your keyboard
- Select Intersection from the left-hand panel of the List Manager dialog (Figure 4)
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SubtitleText | Creating a gene list at the intersection of two criteria |
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AnchorName | Creating an Intersection Gene List |
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- Enter a name for the criteria (Diff Exp and Alt Splice)
- Select OK to close the naming dialog and OK again to close the list creation hint dialog
- Select Save List from the Manage criteria section of the List Manager dialog (Figure 5)
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SubtitleText | Saving a created list criteria |
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AnchorName | Saving Created List |
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- Select Diff Exp and Alt Splice in the List Creator dialog (Figure 6)
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SubtitleText | Selecting list to save in List Creator dialog |
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AnchorName | List Creator dialog |
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- Select OK to save the list
- Select Close to exit the List Manager dialog and view the Diff_Exp_and_Alt_Splice spreadsheet (Figure 7)
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SubtitleText | A list of the differentially expressed and alternatively spliced genes is now available for downstream analysis |
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AnchorName | Viewing Created List |
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This list of differentially expressed and alternatively spliced transcripts will be used later in the next section of the tutorial.
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