After performing exploratory analyses such as PCA, UMAP and t-SNE is is helpful to visualize the results on a scatterplotscatter plot. This can help visually assess the source of variation affecting the results of an experiment, classify cells and select samples for downstream analysis. Here we have a PCA scatterplot scatter plot generated from the analysis of 12 samples from a scRNA sequencing study. The first three most informative PCs are plotted by default and the percentage of variation explained is stated next to each one of them.
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SubtitleText | Example of a 3D PCA scatterplot . |
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AnchorName | Example of a 3D PCA scatterplot |
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![](/download/attachments/60129485/Screenshot%202022-12-20%20at%2015.38.31.png?version=1&modificationDate=1671552052490&api=v2)
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The Configure > Style menu on the left can then be used to color the features in the scatterplot scatter plot based on an attribute (Figure 2). In this case, Figure 3 shows the cells being colored based on their cell-type.
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SubtitleText | Customization menu. |
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AnchorName | Customization menu |
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SubtitleText | PCA scatterplot colored by cell-type. |
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AnchorName | PCA scatterplot colored by cell-type. |
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![](/download/attachments/60129485/Screenshot%202022-12-20%20at%2015.42.22.png?version=1&modificationDate=1671553033932&api=v2)
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