Partek Flow Documentation

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excludeAdditional Assistance

From the GSA the DESeq2 task report, we can browse to any gene in the Chromosome view.

  • Select Click  in the SELM SELENOM row to open Chromosome view (Figure 1)

Numbered figure captions
SubtitleTextBrowsing to a location in Chromosome View
AnchorNameBrowsing to location

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A new tab will open showing SELM SELENOM in the Chromosome view (Figure 2).

 


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SubtitleTextViewing SELM in Chromosome view
AnchorNameSELM in Chromosome view

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Chromosome View shows reference genome, annotation, and data set information together aligned at genomic coordinates. 

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We can add tracks from any data node using Select Tracks.

  • Click Select Select tracks

A pop-up dialog showing the pipeline allows us to choose which data to display as tracks in Chromosome view (Figure 3).

 


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SubtitleTextChoosing tracks to display in Chromosome view
AnchorNameChoosing tracks to display

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  • Select Click Reads pileup under Align Aligned reads on the left-hand side of the dialog 
  • Select Click Display tracks to make the change

The reads pileup track is now included (Figure 4).

 


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SubtitleTextThe reads pileup track shows every read in its aligned position on the reference genome
AnchorNameReads pileup track

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With multiple tracks, it may be useful to pin a track to the top so we can scroll down the reads pileup track without losing sight of the Alignments or RefSeq tracks. 

  • Select Image Removed next to the Alignments track
  • Select Image Removed to pin the track to the top 
  • Repeat for RefSeq
  • Scroll down to view individual reads 

Selecting a read brings up detailed information about the read in the selection details panel (Figure 5).

 

Numbered figure captions
SubtitleTextViewing read information in the Reads pileup track
AnchorNameViewing read information

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To learn more about Chromosome view, please consult the Chromosome View user guide.

 


 

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